Alpha-Tubulin antibody

• Catalog Number: orb345511
• Category: Antibodies
• Description: Alpha-Tubulin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: 1:5,000, IHC: 1:500 - 1:2,000, IF: 1:500 - 1:2,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
• Conjugation: Unconjugated
• UniProt ID: P68363
• NCBI: 17986283
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-
• Note: For research use only
• Application notes: Anti-Tubulin Antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of whole cell lysates from mouse brain (Lane1), rat brain (Lane2), A431 cells (Lane3), Jurkat cells (Lane4), HeLa cells (Lane5) Load: 35 ug per lane using Alpha-Tubulin antibody