TUBA1B Antibody (Biotin)

SKU: orb345885

Description

Tubulin alpha antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, IF: 1:500 - 1:2,000, WB: 1:2,000-1:10,000
• Reactivity: Bovine, Gallus, Goat, Human, Mouse, Rat, Sheep
• Application Notes:
  Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
• Target: TUBA1B
• Purity: Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein. The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
• Conjugation: Biotin

Storage & Handling

• Storage: Store Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot Control Antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Tubulin Antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.61 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-alpha-Tubulin Antibody biotin conjugation, biotin conjugated rabbit anti-α-tubulin antibody, biotin conjugated rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody biotin conjugation, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

ELISA Results of Purified Rabbit Anti-Alpha Tubulin Antibody Biotin Conjugated tested against purified Alpha Tubulin Biotin Conjugated. Each well was coated in duplicate with 0.1 µg of BSA conjugated Alpha Tubulin. The working dilution is 1:11000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).

Quick Database Links

Gene Symbol

TUBA1B

UniProt

P68363