Alpha Tubulin (Acetyl-Lys40) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499276
• Category: Antibodies
• Description: Alpha Tubulin (Acetyl-Lys40) Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: R2A9
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Alpha Tubulin (Acetyl-Lys40) SD(Ac-K)TI
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:1000-4000, IHC-P=1:100-500, IHC-F=1:50-100, ICC/IF=1:25, IF=1:25
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: TUBA4A
• UniProt ID: P68366
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha tubulin 1; alpha-tubulin 1; Detyrosinated al
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-): MCF-7+serum starvation (14hr), (+): MCF-7+ sodium butyrate (50mM, 24hr)+, trichostain A (500 ng/ml, 4 hr), Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 50 kDa, Observed MW: 55 kDa.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:6000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: Mouse brain, 2: Rat brain, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 50 kDa, Observed MW: 55 kDa.

Tissue: Human cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499276.

Tissue: Rat cerebellum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499276.