Alpha Synuclein (pSer129) Antibody (FITC)

SKU: orb612726

Description

Rabbit monoclonal antibody against Alpha Synuclein (Phospho-Ser129) conjugated to FITC

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution range: WB (1:500)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:500 dilution of SMC-600 was sufficient for detection of Alpha Synuclein pSer129 in 10 µg of Mouse Brain by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: J18
• Immunogen: Human alpha synuclein AA 124-134: AYEMP-pS-EEGYQ-Cys
• Target: Alpha Synuclein pSer129
• Purification: Affinity Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Alpha Synuclein, α-Synuclein, SNCA, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Phosphorylated alpha synuclein, Phospho-alpha Synuclein (S129), Alpha-synuclein (phospho S129), Alpha Synuclein (phospho Ser129), Alpha-Synuclein Phospho Ser129, phospho-α-Synuclein (Ser129), Alpha Synuclein phospho Serine 129, Alpha Synuclein phospho Ser 129, Alpha Synuclein pSerine 129, Alpha Synuclein pSer 129, Alpha Synuclein phosphoSer 129, isoform NACP140, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease (autosomal dominant, Lewy body) 4, Parkinson disease familial 1, SYN, Synuclein alpha, Synuclein alpha 140, Synuclein, alpha (non A4 component of amyloid precursor), SYUA_HUMAN

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Tissue: Brain. Species: Mouse. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:10000. Secondary Antibody: anti-rabbit HRP. C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein (pSer129) Monoclonal Antibody, Clone J18. Tissue: iPSC-derived neurons. Species: Human. Primary Antibody: Rabbit Anti-Alpha Synuclein (pSer129) Monoclonal Antibody at 1:1000 for O/N at 4°C. Secondary Antibody: Anti-Rabbit: A488 at 1:1000 for 1 hour at RT. Magnification: 40X. Nuclear stain: Hoechst- 20 min, RT (blue). Actin stain: Phalloidin-647- 20 min, RT (magenta). 4K cells per well. A) negative control; no fibrils added to well. B) 7 days after addition of active recombinant human pre-formed fibrils (Type 1).

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Tissue: Brain. Species: Mouse. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:10000. Secondary Antibody: anti-rabbit HRP. C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (D) 5 ug alpha synuclein PFFs. Inset: PBS (negative control).

Dot Blot analysis using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.

Western Blot analysis of Human Alpha Synuclein showing detection of Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer. Lane 3: 2 ug human alpha synuclein monomer. Lane 4: 0.5 ug human alpha synuclein PFFs. Lane 5: 2 ug human alpha synuclein PFFs. Lane 6: 15 ug human Parkinson's Disease brain lysate. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Western Blot analysis of Mouse Brain showing detection of Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Immunocytochemistry / Immunofluorescence analysis of human iPSC-derived neurons treated with 2.5μg ATTO 594 labeled type I alpha-synuclein pre-formed fibrils for up to 14 days. Cells seeded at 8k cells per well. Green: mouse anti-alpha synuclein (pSer129) monoclonal antibody 1:5000; Red: alpha-synuclein PFFs; Pink: actin; Blue: Hoechst / DNA.

Quick Database Links

Gene Symbol

Alpha Synuclein pSer129

UniProt

P37840

NCBI Gene ID

6622

RefSeq (Protein):NP_000336.1