Alpha Synuclein (pSer129) Antibody (APC)

SKU: orb414135

Description

Rabbit polyclonal antibody against Alpha Synuclein pSer129 conjugated to APC

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); ICC/IF (1:500)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SPC-742 was sufficient for detection of Alpha Synuclein pSer129 in 15 µg of human brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human Alpha Synuclein pSer129 (124-134 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: Alpha Synuclein pSer129
• Molecular Weight: 100, 75, 45, 15 kDa
• Purification: Peptide Affinity Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 0.5mg/mL
• Disclaimer: For research use only

Alternative Names

Alpha Synuclein, α-Synuclein, SNCA, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Phosphorylated alpha synuclein, Phospho-alpha Synuclein (S129), Alpha-synuclein (phospho S129), Alpha Synuclein (phospho Ser129), Alpha-Synuclein Phospho Ser129, phospho-α-Synuclein (Ser129), Alpha Synuclein phospho Serine 129, Alpha Synuclein phospho Ser 129, Alpha Synuclein pSerine 129, Alpha Synuclein pSer 129, Alpha Synuclein phosphoSer 129, isoform NACP140, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease (autosomal dominant, Lewy body) 4, Parkinson disease familial 1, SYN, Synuclein alpha, Synuclein alpha 140, Synuclein, alpha (non A4 component of amyloid precursor), SYUA_HUMAN

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein (pSer129) Polyclonal Antibody. Tissue: Free floating brain sections. Species: Mouse. Fixation: PFA. Primary Antibody: Rabbit Anti-Alpha Synuclein (pSer129) Polyclonal Antibody at 1:500 for overnight at 4°C with gentle agitation. Counterstain: Hoechst. Magnification: 63X. A) Right hemisphere (striatum) injected with alpha synuclein AAV vector. B) Control. Alpha synuclein streaks are visible at injection site, but not control, 4 months after injection.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein (pSer129) Polyclonal Antibody. Species: Mouse. Primary Antibody: Rabbit Anti-Alpha Synuclein (pSer129) Polyclonal Antibody. Phospho serine 129 antibody was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B).

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate at 4 μg/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody at 1:500 for 24 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.

Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Human Alpha Synuclein Monomer (0.5 μg). Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band (s): 100, 75, 45, 30, 16 kDa.

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Quick Database Links

Gene Symbol

Alpha Synuclein pSer129

UniProt

P37840

NCBI Gene ID

6622

RefSeq (Protein):NP_000336.1