Alpha-synuclein Antibody

SKU: orb3167739

Description

Our Anti-Alpha-synuclein sheep polyclonal primary antibody detects human, mouse, and rat Alpha-synuclein, and is affinity-purified. It is validated for use in ELISA, IHC-Frozen, IHC-Paraffin-embedded, WB.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 0.5-2 µg/mL; IHC: 1-4 µg/mL; ICC: 1-4 µg/mL
• Reactivity: Human, Mouse, Rat
• Application Notes:
  IHC: Fixing of proteins on membrane with 0.4% formaldehyde (30 min at room temperature) recommended, see Lee & Kamitani, 2011. Flow Cytometry: 2 µg antibody per ~10^6 cells, methanol-fixed. ICC: 4% formaldehyde-fixed cells.

Key Properties

• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide (CMPVDPDNEAYEMPSEE) of human alpha synuclein protein (aa: 116-131) conjugated to diphtheria toxoid has been used as the immunogen.
• Target: Alpha-synuclein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP

Quick Database Links

Gene Symbol

Alpha-synuclein

UniProt

P37840