alpha Glucosidase II antibody

• Catalog Number: orb556031
• Category: Antibodies
• Description: Rabbit polyclonal antibody to alpha Glucosidase II
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase II. The exact sequence is proprietary.
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 107
• Target: alpha Glucosidase II
• UniProt ID: Q14697
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: glucosidase II alpha subunit , G2AN , GIIA , GLUII
• Note: For research use only
• Application notes: Positive control: Mouse brain , 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , HeLa
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using alpha Glucosidase II antibody

Immunohistochemical staining of SW480 Xenograft using alpha Glucosidase II antibody

Immunohistochemical staining of human breast carcinoma using alpha Glucosidase II antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2), H1299(Lane 3), HeLa S3(Lane 4), HepG2(Lane 5), MOLT4(Lane 6), Raji(Lane 7) using alpha Glucosidase II antibody

Western blot analysis of 293T(Lane 1), A431(Lane 2), H1299(Lane 3), HeLa S3(Lane 4), HepG2(Lane 5), MOLT4(Lane 6), Raji(Lane 7) using alpha Glucosidase II antibody