Alpha Actin Antibody

• Catalog Number: orb624648
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Alpha Actin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: actin, alpha 1, skeletal muscle
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000-1:10000; IP: 1:1000-1:10000; IHC: 1:20-1:200; IF: 1:20-1:200
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: Alpha Actin
• UniProt ID: P68133
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ACTA, ACTA1, ACTA1 skeletal Muscle Actin, actin, A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of HepG2 cells using orb624648 (ACTA1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

IP Result of anti-alpha Actin (IP: orb624648, 4 ug; Detection: orb624648 1:800) with mouse heart tissue lysate 3200 ug.

rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with orb624648 (ACTA1 Antibody) at dilution of 1:4000.

Immunohistochemistry of paraffin-embedded human skeletal muscle slide using orb624648 (ACTA1 Antibody) at dilution of 1:50.