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Alpha Actin Antibody Cocktail / ACTA2 (smooth muscle)

Catalog Number: orb749772

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749772
CategoryAntibodies
DescriptionActin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG2a, kappa
ConjugationUnconjugated
ReactivityHuman, Rat
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenAn N-terminal decapeptide of the alpha smooth muscle isoform of Actin (1A4) and recombinant full-length human protein (ACTA2/791) were used as the immunogen for the ACTA2 antibody cocktail.
UniProt IDP62736
Tested applicationsIF, IHC-P
Dilution rangeImmunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application notesOptimal dilution of the ACTA2 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone Number1A4 + ACTA2/791
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the ACTA2 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Hazard InformationThis ACTA2 antibody cocktail is available for research use only.
NoteFor research use only
Alpha Actin Antibody Cocktail / ACTA2 (smooth muscle)

IHC: Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791).

Alpha Actin Antibody Cocktail / ACTA2 (smooth muscle)

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791).

Alpha Actin Antibody Cocktail / ACTA2 (smooth muscle)

IHC: Formalin-fixed, paraffin-embedded rat lung stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791).

Alpha Actin Antibody Cocktail / ACTA2 (smooth muscle)

IHC: Formalin-fixed, paraffin-embedded rat stomach stained with ACTA2 antibody cocktail (clone 1A4 + ACTA2/791).

Alpha Actin Antibody Cocktail / ACTA2 (smooth muscle)

Immunofluorescent staining of fixed human HeLa cells with ACTA2 antibody cocktail antibody (clone 1A4 + ACTA2/791, green) and NucSpot nuclear stain (red).

Alpha Actin Antibody Cocktail / ACTA2 (smooth muscle)

SDS-PAGE analysis of purified, BSA-free ACTA2 antibody cocktail antibody (clone 1A4 + ACTA2/791) as confirmation of integrity and purity.