Alpha 2C Adrenergic Receptor Antibody: Biotin

• Catalog Number: orb150422
• Category: Antibodies
• Description: Mouse monoclonal to Alpha 2C Adrenergic R (Biotin). The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N330A/80 (Formerly sold as S330A-80)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Synthetic peptide amino acids 442- 462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: Biotin
• MW: 50kDa
• Target: Alpha2C Adrenergic
• Entrez: 152
• UniProt ID: P18825
• NCBI: NP_000674.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Adra 2c antibody, Alpha-2CAR antibody, ADRA2C anti
• Note: For research use only
• Application notes: 1 µg/ml of SMC-435 was sufficient for detection of Alpha2C Adrenergic Receptor in 20 µg of COS cells transiently transfected with HA-tagged Alpha- lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone N330A/80. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody (D) Composite.