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Alpha-1-Anti-Trypsin Antibody Peroxidase Conjugated
Catalog Number: orb344237
| Catalog Number | orb344237 |
|---|---|
| Category | Antibodies |
| Description | Alpha-1-Trypsin antibody (Peroxidase) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Isotype | IgG |
| Conjugation | HRP |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Purity | Anti-alpha-1-Anti-Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]. Cross reactivity against a1-Anti-Trypsin from other sources is unknown. |
| Immunogen | Anti-alpha 1 Anti-Trypsin Antibody was produced by repeated immunizations with native α1-Anti-Trypsin protein from human plasma. |
| UniProt ID | P01009 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Application notes | Anti-Alpha 1 anti-Trypsin Antibody is tested by ELISA and western blot. This product detects a single band of the expected apparent molecular weight (~47 kDa) and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Antibody Type | Primary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | goat anti-Alpha-1-Trypsin Antibody HRP Conjugation Read more... |
| Note | For research use only |
| NCBI | NP_000286.3 |
ELISA Results of Goat Anti-Alpha-1-Anti-Trypsin Peroxidase Conjugated Antibody. Each well was coated in duplicate with 1.0 µg of Alpha-1-Anti-Trypsin. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugated Stabilizer, Goat Anti-Alpha-1-Anti-Trypsin Peroxidase Conjugated Antibody and TMB substrate (p/n orb348651).
Western Blot of Goat Anti-ALPHA 1 ANTI TRYPSIN Antibody Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1 ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10000 for 1.5 hr at RT. Block: orb348637 buffer overnight at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.
Western Blot of Goat-anti-Alpha-1-Anti-Trypsin Antibody. Lane 1: molecular weight. Lane 2: Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35 µg per lane under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Primary antibody: Alpha 1 Anti-Trypsin antibody at 1:400 for overnight at 4°C. Secondary antibody: DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10000 for 30 min at RT in orb348637 30 buffer. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): DyLight 488 Donkey anti-Mouse IgG (blue) and DyLight549 Donkey anti-Rabbit IgG (green) secondary antibodies.
