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Item 1 of 3
ALOXE3 Antibody (Center)
Catalog Number: orb1938981
| Catalog Number | orb1938981 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB17172 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:1000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 80543 Da |
| Target | This ALOXE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-252 amino acids from the Central region of human ALOXE3. |
| UniProt ID | Q9BYJ1 |
| NCBI | NP_001159432.1, NP_067641.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Hydroperoxide isomerase ALOXE3, Epidermis-type lip Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-ALOXE3 Antibody (Center) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HACAT whole cell lysate. Lane 3: A2058 whole cell lysate. Lane 4: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 81 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded human breast tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
Immunohistochemical analysis of paraffin-embedded human skin tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
