ALOX5AP Antibody

SKU: orb1247808

Description

ALOX5AP Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16kDa band observed in lysates of U937 and of Peripheral Blood Mononucleocytes (calculated MW of 18.2kDa according to NP_001620.2). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Spleen shows staining of select splenocytes. Paraffin embedded Human Lung and Small Intestine. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HKVEHESRTQNGR
• Target: ALOX5AP
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALOX5AP, FLAP, arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein, five-lipoxygenase activating protein

orb1247808 (0.1 ug/ml) staining of Human U937 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247808 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247808 (3.75 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247808 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ALOX5AP

UniProt

P20292

RefSeq (Protein):NP_001620.2, NP_001191335.1