ALOX5AP Antibody

• Catalog Number: orb1247808
• Category: Antibodies
• Description: ALOX5AP Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HKVEHESRTQNGR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16kDa band observed in lysates of U937 and of Peripheral Blood Mononucleocytes (calculated MW of 18.2kDa according to NP_001620.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Spleen shows staining of select splenocytes. Paraffin embedded Human Lung and Small Intestine. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ALOX5AP
• UniProt ID: P20292
• NCBI: NP_001191335.1, NP_001620.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ALOX5AP, FLAP, arachidonate 5-lipoxygenase-activat
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16kDa band observed in lysates of U937 and of Peripheral Blood Mononucleocytes (calculated MW of 18.2kDa according to NP_001620.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Spleen shows staining of select splenocytes. Paraffin embedded Human Lung and Small Intestine. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247808 (0.1 ug/ml) staining of Human U937 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247808 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247808 (3.75 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247808 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.