ALOX15/15-Lipoxygenase Antibody

SKU: orb1535527

Description

ALOX15/15-Lipoxygenase Antibody

Images & Validation

• Tested Applications: FC, IF, IHC, IHC-P, WB
• Dilution range: Flo (1:100), IF (1:100), IHC, IHC-P (10 µg/ml), WB (1:2000)
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 7H6
• Immunogen: Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
• Target: ALOX15 / 15-Lipoxygenase
• Purification: Protein A/G purified from tissue culture supernatant.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ALOX15, 15-lipoxygenase, 15-LOX, 15-lipooxygenase-1, 15-LO-1, 15LOX-1, 15-LOX-1, LOG15, Arachidonate 15-lipoxygenase

Anti-ALOX15 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALOX15 antibody, and then analyzed by flow cytometry.

Quick Database Links

Gene Symbol

ALOX15 / 15-Lipoxygenase