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Catalog Number | orb1535527 |
---|---|
Category | Antibodies |
Description | ALOX15/15-Lipoxygenase Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 7H6 |
Tested applications | FC, IF, IHC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2b |
Immunogen | Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell. |
Concentration | 1 mg/ml |
Dilution range | Flo (1:100), IF (1:100), IHC, IHC-P (10 µg/ml), WB (1:2000) |
Conjugation | Unconjugated |
Target | ALOX15 / 15-Lipoxygenase |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin |
Alternative names | ALOX15, 15-lipoxygenase, 15-LOX, 15-lipooxygenase- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-ALOX15 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALOX15 antibody, and then analyzed by flow cytometry.