ALOX12B Rabbit Polyclonal Antibody

SKU: orb2953180

Description

Anti-ALOX12B Polyclonal Antibodyis a rabbit polyclonal antibody detecting ALOX12B in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat. Highlights Affinity Purified: Minimal background and high purity for reliable results. Multi-Application: Validated across multiple applications. Multi-Species: Cross-reactive for translational research.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E. coli - derived recombinant Human ALOX12B (Leu479-Ile701).
• Purification: Purified by antigen affinity column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALOX12B, Epidermis-type lipoxygenase 12, Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALOX12B (orb2953180) at a concentration of 28 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALOX12B was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Quick Database Links

UniProt

O75342