Aloesin

SKU: orb1220744

Description

Aloesin is a C-glycosylated chromone compound found in aloe (Liliaceae) that inhibits tyrosinase (IC50 = 0.9 mM) an enzyme responsible for catalyzing the first step of the conversion of tyrosine to melanin.

Key Properties

• CAS Number: 30861-27-9
• MW: 394.37
• Purity: >98% (HPLC)
• Formula: C₁₉H₂₂O₉
• SMILES: CC(=O)Cc1cc(=O)c2c(C)cc(O)c([C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)c2o1
• Target: Tyrosinase
• Solubility: DMSO:78 mg/mL (197.78 mM)

Bioactivity

• In Vivo:
  Aloesin (injection; 20 mg/kg or 40 mg/kg; once daily; 7 w) inhibits tumor growth in a Xenograft model of ovarian cancer. Animal model: 6-week-old athymic nude mice injected with SKOV3 cells. Dosage: 20 mg/kg or 40 mg/kg. Administration: Injection; 20 mg/kg or 40 mg/kg; once daily; 7 weeks. Result: Reduced volumes and average weights of dissected tumors significantly compared with the control group.
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• In Vitro:
  Aloesin (0-40 μM, 24, 48, and 72 h) inhibits ovarian cancer cell growth in a dose- and time-dependent manner. Aloesin (0-10 μM; 48 h) arrest the cell cycle at S-phase in a dose-dependent manner in SKOV3 cells. Aloesin (0-10 μM; 48 h) promotes cell apoptosis in SKOV3 cells. Aloesin (0-10 μM; 48 h) inhibits the phosphorylation of the MAPK signaling pathway. Cell Viability Assay Cell line: MCF10A, T47D, MCF7, MDA-MB-468, and MDA-MB-231 cells. Concentration: 0, 2.5, 5, 10, 20, and 40 μM Incubation time: 24, 48, and 72 h. Result: Exhibited a concentration-dependent and time-dependent killing of diverse ovarian cancer cell lines. Cell Viability Assay Cell line: SKOV3 cells. Concentration: 2.5-10 μM Incubation time: 48 hours. Result: Showed a significantly potent toxic effect with an IC50 value of around 5 μM. Cell Cycle Analysis Cell line: SKOV3 cells. Concentration: 0, 2.5, 5, and 10 μM Incubation time: 48 h. Result: Decresed the percentage of cells in the G2/M phase gradually with increasing doses, increased the percentages of cells in the S-phase. Downregulated the levels of the S-G2/M-related proteins cyclin A, CDK2, and cyclin D1.Apoptosis Analysis Cell line: SKOV3 cells. Concentration: 0, 2.5, 5, and 10 μM Incubation time: 48 h. Result: Detected significant proteolytic cleavage of caspase-3, caspase-9, and PARP1.Increased the expression of Bax and conversely decreased Bcl-2. Western blot analysis. Cell line: SKOV3 cells. Concentration: 0, 2.5, 5, and 10 μM Incubation time: 48 h. Result: Decreased phosphorylated levels of MEK, ERK, MAPK, and JNK.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Tyrosinase