ALKBH6 Antibody (Center)

SKU: orb1938303

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB28929
• Immunogen: This ALKBH6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the Central region of human ALKBH6. Antigen Region: 116-145 aa.
• Target: ALKBH6 (HGNC:28243)
• Molecular Weight: 26483 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6, 11411-, Alkylated DNA repair protein alkB homolog 6, ALKBH6, ABH6

ALKBH6 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the ALKBH6 antibody detected the ALKBH6 protein (arrow).

ALKBH6 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ALKBH6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ALKBH6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Anti-ALKBH6 Antibody (Center) at 1:1000 dilution + MOLT-4 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ALKBH6 (HGNC:28243)

UniProt

Q3KRA9

RefSeq (Protein):NP_116267.3