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ALKBH5 Antibody (Center)

Catalog Number: orb1933179

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1933179
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
TargetThis ALKBH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the Central region of human ALKBH5.
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityMouse, Rat
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
UniProt IDQ6P6C2
MW44256 Da
Tested applicationsFC, IHC-P, WB
Dilution rangeWB: 1:500, WB: 1:2000, IHC-P: 1:100, FC: 1:25
Antibody TypePrimary Antibody
Clone NumberRB38474
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesRNA demethylase ALKBH5, 11411-, Alkylated DNA repa
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NoteFor research use only
NCBINP_060228.3
ALKBH5 Antibody (Center)

Staining ALKBH5 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

ALKBH5 Antibody (Center)

Overlay histogram showing U-2 OS cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

ALKBH5 Antibody (Center)

Anti-ALKBH5 Antibody (Center) at 1:2000 dilution + 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ALKBH5 Antibody (Center)

All lanes: Anti-ALKBH5 Antibody (Center) at 1:500 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.