ALG6 Antibody

SKU: orb750526

Description

ALG6 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:45,000, IHC: 4µg/mL, WB: 1:1000
• Reactivity: Human
• Application Notes:
  Anti-ALG6 antibody has been tested in ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 58kDa in size corresponding to ALG6 protein by Western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of human ALG6 protein, conjugated to KLH.
• Target: ALG6
• Purity: ALG6 Antibody antiserum is directed against human ALG6. A 58 kDa band is detected when assayed by immunoblot. Cross reactivity is expected against human ALG6 protein based on a 100% homology of the amino acid sequence of the immunogen between rat, mouse, drosophila, and pongo abelii.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 80 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ALG6 Antibody, rabbit anti-ALG-6 Antibody, Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase, Asparagine-linked glycosylation protein 6 homolog, Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase, Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase, ALG6 Antibody, My046

Immunohistochemistry with anti-ALG6 antibody showing ALG6 staining in the squamous epithelium of the uterine cervix as well as in inflammatory elements diffused in the stroma of human cervical cancer tissue at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH6.2 and then incubated with rabbit anti-human ALG6 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).

Western Blot of Rabbit anti-ALG6 antibody. Lane 1: HeLa Whole Cell lysate (p/n orb348668). Lane 2: MOLT4 Whole Cell lysate (p/n orb348696). Lane 3: K-562 Whole Cell lysate (p/n orb348692). Load: 10 µg per lane. Primary antibody: ALG6 antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: TBS-Casein overnight at 4°C. Predicted/Observed size: 58 kDa ALG-6.

Quick Database Links

Gene Symbol

ALG6

UniProt

Q9Y672