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Alfy/WDFY3 Antibody

SKU: orb1248114

Description

Alfy/WDFY3 Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

Tested ApplicationsELISA, WB
ReactivityMouse
Predicted ReactivityBovine, Human, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 400kDa band observed in Mouse Brain lysates (calculated MW of 395kDa according to NP_055806.2). Data obtained from anonymous customer. The lower molecular weight bands are non-specific. We call for caution when used for other assays than Western blot. Recommended concentration: 4-6ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-SPERSTRTQQKEFQT
TargetAlfy / WDFY3
Molecular WeightApprox 400kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

WDFY3, WD repeat and FYVE domain containing 3, ALFY, ZFYVE25, WD repeat and FYVE domain-containing protein 3, autophagy-linked FYVE protein

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Alfy/WDFY3 Antibody

orb1248114 (5 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Alfy/WDFY3 Antibody (orb1248114)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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