ALDOC Rabbit Polyclonal Antibody

SKU: orb2953116

Description

Anti-ALDOC Polyclonal Antibodyis a rabbit polyclonal antibody detecting Brain-type aldolase in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat. Highlights Affinity Purified: Minimal background and high purity for reliable results. Multi-Application: Validated across multiple applications. Multi-Species: Cross-reactive for translational research.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E. coli - derived recombinant Human ALDOC (Asp34-Ser281).
• Purification: Purified by antigen affinity column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-type aldolase, ALDOC, ALDC, Fructose-bisphosphate aldolase C

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALDOC (orb2953116) at a concentration of 24 µg/mL overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALDOC was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Western blot analysis was performed using anti-ALDOC polyclonal antibody at 1 µg/mL on various samples. Lane 1: MCF7 cell lysate, Lane 2: LO2 cell lysate, Lane 3: k562 cell lysate, Lane 4: Jurkat cell lysate, Lane 5: Mouse brain lysate, Lane 6: Mouse kidney lysate, Lane 7: Mouse muscle lysate, Lane 8: Mouse heart lysate.

Quick Database Links

UniProt

P09972