ALDOC Antibody (C-term)

• Catalog Number: orb1927313
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Target: Purified His-tagged ALDOC protein was used to produced this monoclonal antibody.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG3
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: P09972
• MW: 39456 Da
• Tested applications: WB
• Dilution range: WB: 1:1000, WB: 1:2000, FC: 1:25
• Clone Number: 859CT9.5.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Fructose-bisphosphate aldolase C, Brain-type aldol
• Note: For research use only
• NCBI: NP_005156.1

ALDOC Antibody (C-term) western blot analysis in HL-60, 293 cell line and mouse brain, spleen lysates (35μg/lane).This demonstrates the ALDOC antibody detected the ALDOC protein (arrow).

All lanes: Anti-ALDOC Antibody (C-term) at 1:2000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: 293T/17 whole cell lysate. Lane 3: mouse brain lysate. Lane 4: rat brain lysate. Lane 5: mouse spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HL-60 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG3 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.