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Catalog Number | orb1931515 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB14945 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Rabbit |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 39420 Da |
Target | This ALDOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-331 amino acids from the C-terminal region of human ALDOA. |
UniProt ID | P04075 |
NCBI | NP_908932.1, NP_000025.1, NP_908930.1, NP_001230106.1, NP_001121089.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Fructose-bisphosphate aldolase A, Lung cancer anti Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-ALDOA Antibody (C-term) in mouse liver tissue lysates (35 ug/lane). ALDOA (arrow) was detected using the purified Pab.
Western blot analysis of lysates from MCF-7, Hela cell line and human skeletal muscle tissue lysate (from left to right), using ALDOA Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |