ALDH6A1 Antibody

SKU: orb1788203

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Immunogen: This ALDH6A1 antibody is generated from mouse immunized with ALDH6A1 recombinant protein. Antigen Region: 104-523 aa.
• Target: ALDH6A1 (HGNC:7179)
• Molecular Weight: 57840 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALDH6A1; MMSDH; Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; Aldehyde dehydrogenase family 6 member A1

Western blot analysis of lysates from HepG2, T47D cell line (from left to right), using ALDH6A1 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded H.colon section using ALDH6A1 Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Quick Database Links

Gene Symbol

ALDH6A1 (HGNC:7179)

UniProt

Q02252

RefSeq (Protein):NP_005580.1