ALD2 Antibody

SKU: orb750571

Description

ALD2 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Yeast
• Application Notes:
  Anti-Aldehyde Dehydrogenase has been tested by western blot and is suitable to be assayed against 1.0 ug of Aldehyde Dehydrogenase [Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Aldehyde Dehydrogenase [Yeast]
• Target: ALD2
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Aldehyde Dehydrogenase [Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase, [NAD(P)+] 1, ALD2, ALD5

Western Blot of Rabbit anti-Aldehyde Dehydrogenase (yeast) Antibody. Lane 1: Aldehyde Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Aldehyde Dehydrogenase (yeast) Antibody at 1:500 overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat anti-Rabbit IgG secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Aldehyde Dehydrogenase (yeast).

Quick Database Links

Gene Symbol

ALD2

UniProt

P47771

RefSeq (Protein):NP_013893.1