ALD2 Antibody

SKU: orb344801

Description

Aldehyde Dehydrogenase antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Reactivity: Yeast
• Application Notes:
  Anti-Aldehyde Dehydrogenase has been tested by western blot. Assays should be optimized by the end user for specific conditions for reactivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Aldehyde Dehydrogenase [Yeast]
• Target: ALD2
• Purity: Anti-Aldehyde Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase [NAD(P)+] 1, ALD2, ALD5, ALD

Western Blot Results of Rabbit Anti-Aldehyde Dehydrogenase Antibody. Lane 1: Molecular Weight Marker. Lane 2: Aldehyde Dehydrogenase. Primary Antibody: Anti-Aldehyde Dehydrogenase at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG (p/n orb347654) at 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 30 min at RT. Expect MW: ~55kDa.

Quick Database Links

Gene Symbol

ALD2

UniProt

P47771

RefSeq (Protein):NP_013893.1