Aldehyde Dehydrogenase Antibody

SKU: orb344800

Description

Aldehyde Dehydrogenase antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:2,000
• Reactivity: Yeast
• Application Notes:
  Anti-Aldehyde Dehydrogenase has been tested by western blot. Assays should be optimized by the end user for specific conditions for reactivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Aldehyde Dehydrogenase [Yeast]
• Purity: Anti-Aldehyde Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast]. Cross reactivity against Aldehyde Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase [NAD(P)+] 1, ALD2, ALD5, ALD

Western Blot Results of Rabbit Anti-Aldehyde Dehydrogenase Antibody. Lane 1: Molecular Weight Marker. Lane 2: Aldehyde Dehydrogenase. Primary Antibody: Anti-Aldehyde Dehydrogenase at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG (p/n orb347654) at 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 30 min at RT. Expect MW: ~55kDa.

Quick Database Links

UniProt

P47771

RefSeq (Protein):NP_013893.1