Alas1 Rabbit mAb [KD Validated] Antibody

SKU: orb1951662

Description

5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells.

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB 1:500~1:2000 ICC/IF 1:50~1:200
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Alas1
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALAS1; ALAS-H; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; ALAS3; ALASH;

All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.

Western blot analysis of Alas1 expression in HepG2 cell lysate.

Quick Database Links

UniProt

P13196