AKT2 Antibody

SKU: orb344467

Description

AKT2 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:1000 - 1:10,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  Anti-AKT2 Antibody has been tested by western blot and is suitable for ELISA and immunohistochemistry. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 16C8.E4.G7.E9
• Immunogen: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
• Target: AKT2
• Purity: Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Mouse anti-AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

Western Blot of Mouse anti-AKT2 antibody. MW ladder (opal pre-stained). Lane 1: His-AKT1. Lane 2: His-AKT2. Lane 3: His-AKT3. Load: 0.05 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Rb-a-ms IgG (p/n orb347506) at 1:40000 for 30 min at RT. Block: BlockOut p/n (orb348637) overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2. Other band(s): none.

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751

RefSeq (Protein):NP_001617.1