AKT2 Antibody (Center)

SKU: orb37319

Description

Rabbit polyclonal antibody to AKT2

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IF/ICC: 1:10-50, FACS: 1:10-50, IHC-P: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB3026
• Immunogen: Synthetic Peptide
• Target: AKT2
• Molecular Weight: 55769
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-"RAC-beta serine/threonine protein kinase antibody, anti-RAC-PK-beta antibody, anti-Protein kinase Akt-2 antibody

AKT2 Antibody (N108) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the AKT2 antibody detected the AKT2 protein (arrow).

Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2).

AKT2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of AKT2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

AKT2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751

RefSeq (Protein):NP_001617.1