AKT1/2/3 Antibody (Center)

• Catalog Number: orb1927101
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB43841
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse, Other
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 55686 Da
• Target: This AKT1/2/3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 278-312 amino acids from the Central region of human AKT1/2/3.
• UniProt ID: P31749
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RAC-alpha serine/threonine-protein kinase, Protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. prostate carcinoma section using AKT1/2/3 Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Jurkat, PC-3, C6 cell line (from left to right), using AKT1/2/3 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.