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Catalog Number | orb534601 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to AKT1 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | AKT1/2491 |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | IgG2b |
Immunogen | Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary) |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Dilution range | IHC-P: 1-2 μg/ml for 30 min at RT |
Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Conjugation | Unconjugated |
MW | 62kDa |
Target | AKT1 |
Entrez | 207 |
UniProt ID | P31749 |
Expression System | HEK293 cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Alternative names | Oncogene AKT1; PKB; PKB-ALPHA; PRKBA; Protein Kina Read more... |
Note | For research use only |
Application notes | Positive Control: MCF-7 cells. PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma |
Expiration Date | 12 months from date of receipt. |
ELISA, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |