AKT1 (phospho S473) Antibody [H4]

• Catalog Number: orb1274078
• Category: Antibodies
• Description: AKT1 (phospho S473) Antibody [H4]
• Target: AKT1
• Clonality: Monoclonal
• Isotype: Recombinant Monoclonal Rabbit IgG / light chain kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Sheep
• Concentration: batch dependent
• Buffer/Preservatives: 49% PBS, 50% Glycerol, 0.05% rAlbumin, pH 7.2, 0.02% NaN3
• Purification: Protein A purified
• Immunogen: Synthetic peptides derived from human AKT1 (RPHFPQFsYSASGTA)
• UniProt ID: P31749
• Tested applications: IP, WB
• Application notes: WB, IP dilution (1:1000 – 1: 5000).
• Antibody Type: Primary Antibody
• Clone Number: H4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
• Research Area: Cancer Biology, Metabolism Research, Signal Transd
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Antibody: orb1274078 at 1/1000 dilution Sample: NIH-3T3 cell lysate treated with PDGF and without PDGF at 30 ug/lane Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution Predicted band size: 56/60 kDa Observed band size: 66 kDa Gel concentration: 4-20%

Antibody: orb1274078 at 1/1000 dilution Sample: Lane 1: 1 ug of PDGF treated NIH-3T3 cell lysate. Lane 2: 500 ug of PDGF treated NIH-3T3 cell lysate were incubated with anti-phosop-AKT1 antibody coupled to magnetic beads, Lane 3: Negative control 500 ug of PDGF treated NIH-3T3 cell lysate were ncubated with negative control antibody coupled magnetic beads Secondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilution Predicted band size: 56/66 kDa Observed band size: 66 kDa Gel concentration: 4-20%