AKT1 Antibody

SKU: orb1274078

Description

AKT1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IP, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Sheep
• Application Notes:
  WB, IP dilution (1:1000 – 1: 5000).

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Recombinant Monoclonal Rabbit IgG / light chain kappa
• Clone No.: H4
• Immunogen: Synthetic peptides derived from human AKT1 (RPHFPQFsYSASGTA)
• Target: AKT1
• Purification: Protein A purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 49% PBS, 50% Glycerol, 0.05% rAlbumin, pH 7.2, 0.02% NaN3
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA

Antibody: orb1274078 at 1/1000 dilution Sample: NIH-3T3 cell lysate treated with PDGF and without PDGF at 30 ug/lane Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution Predicted band size: 56/60 kDa Observed band size: 66 kDa Gel concentration: 4-20%

Antibody: orb1274078 at 1/1000 dilution Sample: Lane 1: 1 ug of PDGF treated NIH-3T3 cell lysate. Lane 2: 500 ug of PDGF treated NIH-3T3 cell lysate were incubated with anti-phosop-AKT1 antibody coupled to magnetic beads, Lane 3: Negative control 500 ug of PDGF treated NIH-3T3 cell lysate were ncubated with negative control antibody coupled magnetic beads Secondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilution Predicted band size: 56/66 kDa Observed band size: 66 kDa Gel concentration: 4-20%

Quick Database Links

Gene Symbol

AKT1

UniProt

P31749