AKT1 APC Antibody

• Catalog Number: orb344558
• Category: Antibodies
• Description: AKT1 APC antibody (APC)
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: APC
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: None
• Purity: Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
• Immunogen: Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
• UniProt ID: P31749
• Tested applications: DOT, FC
• Dilution range: FC: 1.0 µg/mL
• Application notes: Anti-AKT1 APC Antibody is tested for Flow Cytometry This antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
• Antibody Type: Primary Antibody
• Clone Number: 5E5.F5.D7
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Alternative names: mouse anti-AKT1 antibody APC conjugation, allophyc
• Note: For research use only
• NCBI: NP_001014431.1
• Expiration Date: 12 months from date of receipt.

Dot Blot of AKT1 Allophycocyanin Conjugated Monoclonal Antibody. Antigen: AKT1 non-phosphorylated. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: AKT1 Allophycocyanin Conjugated Monoclonal Antibody at 1:1000 in orb348637 for 60 min at RT. Block: orb348637 for 60 min at RT.

Flow Cytometry of Mouse anti-AKT1 antibody. Cells: LNCap Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT1 antibody at 1.0 µg/ml for 20 min at 4°C.