AKT1 Antibody (C-term T450)

SKU: orb1438858

Description

Rabbit polyclonal antibody to AKT1 (T450).

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB29255
• Immunogen: Synthetic Peptide
• Target: AKT1
• Molecular Weight: 55686
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC

AKT1 Antibody (C-term T450) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AKT1 Antibody (C-term T450) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of AKT1 Antibody (C-term T450) for immunohistochemistry. Clinical relevance has not been evaluated.

AKT1 Antibody (pT450) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).

AKT1 Antibody (pT450) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).

Confocal immunofluorescent analysis of AKT1 Antibody (C-term T450) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

AKT1

UniProt

P31749

RefSeq (Protein):NP_001014431.1, NP_001014432.1, NP_005154.2