AKT1 Antibody (C-term)

SKU: orb1929633

Description

AKT1 Antibody (C-term)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Immunology & inflammation, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:1000, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 55686 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PKB, RAC

AKT1 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of hAKT1-D453 in Y79 cell line lysates (35 ug/lane).AKT1 (arrow) was detected using the purified Pab.

AKT1 Antibody (C-term) western blot analysis in T47D cell line lysates (35 ug/lane). This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).

Western blot analysis of AKT1 (arrow) using rabbit polyclonal hAKT1-D453. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2).

Quick Database Links

UniProt

P31749