Akt (phospho-T308) antibody

• Catalog Number: orb345379
• Category: Antibodies
• Description: Akt (phospho-T308) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-AKT pT308 polyclonal antibody was produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding threonine 308 of human AKT1 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:15,000, FC: User Optimized, IHC: 1:200, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: AKT phospho T308 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
• Conjugation: Unconjugated
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store AKT phospho T308 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-AKT pT308 Antibody, AKT1 phospho T308,
• Note: For research use only
• Application notes: AKT antibody is phospho specific for pT308 and is tested for western blotting, Immunohistochemistry, dot blot, and ELISA. This antibody is suitable for flow cytometry.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GST tagged AKT1 un-active recombinant protein (Lane1), GST tagged AKT1 active recombinant protein (Lane2) using Akt (phospho-T308) antibody