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Akt (Phospho-Ser473) polyclonal antibody
Description
Research Area
Images & Validation
−
| Tested Applications | IF, IP, WB |
|---|---|
| Dilution Range | WB: 1:5000~1:10000, IF: 1:50~1:200, IP: 1:50~1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic phosphopeptide derived from human Akt around the phosphorylation site of Serine 473. |
| Molecular Weight | ~ 60 kDa |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Protocol Information
Akt (Phospho-Ser473) polyclonal antibody (orb1145686)
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