Akt (Phospho-Ser473) Antibody

SKU: orb222951

Description

Akt (Phospho-Ser473) Antibody is an unconjugated polyclonal antibody. It is suitable for use in IHC, WB. It exhibits reactivity with Human, Mouse, Rat.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500-1:3000, IHC-P: 1:50-1:100, ELISA: 1:5000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: C-terminal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti AKT 1 antibody, anti AKT antibody, anti AKT1 antibody, anti C AKT antibody, anti Oncogene AKT1 antibody, anti PKB antibody, anti PKB-ALPHA antibody, anti PRKBA antibody, anti Protein Kinase B Alpha antibody, anti Protein kinase B antibody, anti Proto-oncogene c-Akt antibody, anti RAC Alpha antibody, anti RAC antibody, anti RAC PK Alpha antibody, anti RAC Serine/Threonine Protein Kinase antibody, anti RAC-alpha serine/threonine-protein kinase antibody, anti RAC-PK-alpha antibody, anti vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody

Western blot analysis of HeLa cells using Akt(Phospho-Ser473) Antibody.

Immunohistochemical analysis of breast carcinoma using Akt (Phospho-Ser473) Antibody.

Quick Database Links

UniProt

P31749, Q9Y243, P31751

Ma, Lin et al. Baicalein Protects Rats with Diabetic Cardiomyopathy Against Oxidative Stress and Inflammation Injury via Phosphatidylinositol 3-Kinase (PI3K)/AKT Pathway Med Sci Monit, 24, 5368-5375 (2018)

PubMed: 30070262

Applications

WB

Reactivity

Rat

Tao, Haitao et al. Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway Clin Interv Aging, 15, 373-385 (2020)

PubMed: 32214804

Applications

WB

Reactivity

Rat

Xu, Dongsheng et al. miR-10a overexpression aggravates renal ischemia-reperfusion injury associated with decreased PIK3CA expression BMC Nephrol, 21, 248 (2020)

PubMed: 32611384

Applications

WB

Reactivity

Rat