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Item 1 of 5
Item 1 of 5
| Catalog Number | orb127667 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to AKT (phospho-Ser473) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | C-terminal |
| Concentration | 1mg/ml |
| Dilution range | WB: 1:500-1:3000, ELISA: 1:1000, IHC-P: 1:50-1:200 |
| Conjugation | Unconjugated |
| UniProt ID | P31749 |
| Alternative names | anti AKT 1 antibody, anti AKT antibody, anti AKT1 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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Western blot analysis of extracts from K562 using Akt (phospho-Ser473) antibody
_antibody_orb127667_wb_2.jpg)
Western blot analysis of extracts from JK cells using Akt (phospho-Ser473) antibody
_antibody_orb127667_ihc_p_1.jpg)
Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human breast carcinoma tissue using Akt (phospho-Ser473) antibody
_antibody_orb127667_if_1.jpg)
Confocal immunofluorescence analysis of A549 cells using Akt (phospho-Ser473) antibody
Western blot analysis of extracts from K562 cells using CIITA antibody (see review by T. Sharma)
- Item 1 of 7
AKT (Phospho-Ser473) antibody [orb229611]
ELISA, ICC, IF, IHC-P, WB
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl, 200 μl - Item 1 of 2
AKT (Phospho-Ser473) antibody [orb222951]
IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μg, 100 μg, 200 μg, 25 μg - Item 1 of 5
Akt1 (phospho-Ser473) antibody [orb526646]
FC, ICC, IHC-P, WB
Human, Mouse
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl, 25 μl - Item 1 of 3
Akt (Phospho-Ser473) Antibody [orb14785]
IF, IHC, WB
Human, Mouse, Rat
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl - Item 1 of 3
AKT (phospho-Ser473) antibody [orb506119]
IHC-P
Bovine, Canine, Porcine, Rabbit, Sheep
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
100 μl, 200 μl, 50 μl
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AKT (phospho-Ser473) antibody (orb127667)
For western, I used 1:1000 dilution and prepared the antibody in 3% BSA. It worked efficiently. A single band at the expected size was obtained. For IF, I used 1:50 dilution in % BSA solution prepared in 1X PBS. For IP, I used 2ug for 500 ug of whole cell lysate of the MCF-7 cell line. It's a very good antibody and provides reproducible results.
- Tanisha Sharma
Applications
IF,IP,WB
Rating
- 5 stars