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Akt (Phospho-Ser473) Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | WB: 1:500-1:3000, ELISA: 1:1000, IHC-P: 1:50-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | C-terminal |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of extracts from K562 using Akt (phospho-Ser473) antibody
_antibody_orb127667_wb_2.jpg)
Western blot analysis of extracts from JK cells using Akt (phospho-Ser473) antibody
_antibody_orb127667_ihc_p_1.jpg)
Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human breast carcinoma tissue using Akt (phospho-Ser473) antibody
_antibody_orb127667_if_1.jpg)
Confocal immunofluorescence analysis of A549 cells using Akt (phospho-Ser473) antibody

Western blot analysis of extracts from K562 cells using CIITA antibody (see review by T. Sharma)
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Akt (Phospho-Ser473) Antibody (orb127667)
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For western, I used 1:1000 dilution and prepared the antibody in 3% BSA. It worked efficiently. A single band at the expected size was obtained. For IF, I used 1:50 dilution in % BSA solution prepared in 1X PBS. For IP, I used 2ug for 500 ug of whole cell lysate of the MCF-7 cell line. It's a very good antibody and provides reproducible results.

































