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Akt (Phospho-Ser473) Antibody

Catalog Number: orb127667

DispatchUsually dispatched within 5-10 working days
$ 110.00
Catalog Numberorb127667
CategoryAntibodies
DescriptionRabbit polyclonal antibody to AKT (phospho-Ser473)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
ImmunogenC-terminal
Concentration1mg/ml
Dilution rangeWB: 1:500-1:3000, ELISA: 1:1000, IHC-P: 1:50-1:200
ConjugationUnconjugated
UniProt IDP31749
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti AKT 1 antibody, anti AKT antibody, anti AKT1
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NoteFor research use only
Expiration Date12 months from date of receipt.
Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from K562 using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from JK cells using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human breast carcinoma tissue using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Confocal immunofluorescence analysis of A549 cells using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from K562 cells using CIITA antibody (see review by T. Sharma)

  • Phospho-AKT (Ser473) Rabbit Polyclonal Antibody [orb11293]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Gallus, Porcine, Rabbit, Sheep

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • Phospho-Akt1 (Ser473) Recombinant Rabbit Monoclonal Antibody [orb526646]

    FC,  ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Mouse

    Human, Mouse, Rat

    Rabbit

    Recombinant

    Unconjugated

    50 μl, 100 μl, 25 μl
  • Phospho-AKT (Ser473) Mouse Monoclonal Antibody [orb506119]

    IF,  IHC-Fr,  IHC-P

    Bovine, Canine, Porcine, Rabbit, Sheep

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • Akt (phospho Ser473) Polyclonal Antibody [orb1415484]

    IF,  IHC-P,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • Akt (Phospho-Ser473) Antibody [orb222951]

    IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg, 200 μg, 25 μg
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Akt (Phospho-Ser473) Antibody (orb127667)

For western, I used 1:1000 dilution and prepared the antibody in 3% BSA. It worked efficiently. A single band at the expected size was obtained. For IF, I used 1:50 dilution in % BSA solution prepared in 1X PBS. For IP, I used 2ug for 500 ug of whole cell lysate of the MCF-7 cell line. It's a very good antibody and provides reproducible results.

- Tanisha Sharma

Applications

IF,IP,WB

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