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Catalog Number | orb127667 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to AKT (phospho-Ser473) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | C-terminal |
Concentration | 1mg/ml |
Dilution range | WB: 1:500-1:3000, ELISA: 1:1000, IHC-P: 1:50-1:200 |
Conjugation | Unconjugated |
UniProt ID | P31749 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti AKT 1 antibody, anti AKT antibody, anti AKT1 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts from K562 using Akt (phospho-Ser473) antibody
Western blot analysis of extracts from JK cells using Akt (phospho-Ser473) antibody
Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human breast carcinoma tissue using Akt (phospho-Ser473) antibody
Confocal immunofluorescence analysis of A549 cells using Akt (phospho-Ser473) antibody
Western blot analysis of extracts from K562 cells using CIITA antibody (see review by T. Sharma)
FC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
IF, IHC-Fr, IHC-P | |
Bovine, Canine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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For western, I used 1:1000 dilution and prepared the antibody in 3% BSA. It worked efficiently. A single band at the expected size was obtained. For IF, I used 1:50 dilution in % BSA solution prepared in 1X PBS. For IP, I used 2ug for 500 ug of whole cell lysate of the MCF-7 cell line. It's a very good antibody and provides reproducible results.
- Tanisha Sharma
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