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Akt (Phospho-Ser473) Antibody

SKU: orb127667

Description

Akt (Phospho-Ser473) Antibody is an unconjugated polyclonal antibody. It is suitable for use in IF, IHC, WB. It exhibits reactivity with Human, Mouse, Rat.

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC, WB
Dilution RangeWB: 1:500-1:3000, ELISA: 1:1000, IHC-P: 1:50-1:200
ReactivityHuman, Mouse, Rat

Key Properties

HostRabbit
ClonalityPolyclonal
ImmunogenC-terminal
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti AKT 1 antibody, anti AKT antibody, anti AKT1 antibody, anti C AKT antibody, anti Oncogene AKT1 antibody, anti PKB antibody, anti PKB-ALPHA antibody, anti PRKBA antibody, anti Protein Kinase B Alpha antibody, anti Protein kinase B antibody, anti Proto-oncogene c-Akt antibody, anti RAC Alpha antibody, anti RAC antibody, anti RAC PK Alpha antibody, anti RAC Serine/Threonine Protein Kinase antibody, anti RAC-alpha serine/threonine-protein kinase antibody, anti RAC-PK-alpha antibody, anti vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody

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Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from K562 using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from JK cells using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human breast carcinoma tissue using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Confocal immunofluorescence analysis of A549 cells using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from K562 cells using CIITA antibody (see review by T. Sharma)

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

Akt (Phospho-Ser473) Antibody (orb127667)

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Applications: IP, IF, WB|Reactivity: Human

For western, I used 1:1000 dilution and prepared the antibody in 3% BSA. It worked efficiently. A single band at the expected size was obtained. For IF, I used 1:50 dilution in % BSA solution prepared in 1X PBS. For IP, I used 2ug for 500 ug of whole cell lysate of the MCF-7 cell line. It's a very good antibody and provides reproducible results.

- Tanisha SharmaNCCS

Available Sizes

Select a size below

25 μg
$ 110.00
50 μg
$ 170.00
100 μg
$ 270.00
200 μg
$ 390.00
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