Akt (phospho-S473) antibody

• Catalog Number: orb344456
• Category: Antibodies
• Description: Akt (phospho-S473) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 17F6.B11
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, FC: User Optimized, IHC: 20 µg/ml, IF: 1:500 - 1:3,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-AKT pS473 Antibody, RAC-PK-alpha, Prote
• Note: For research use only
• Application notes: This monoclonal antibody is tested in ELISA, immunohistochemistry, immunofluorescent microscopy, and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human prostate tissue using Akt (phospho-S473) antibody

Western blot analysis of unstimulated NIH/3T3 lysates using Akt (phospho-S473) antibody

Immunofluorescence analysis of A431 cells using Akt (phospho-S473) antibody

Immunofluorescence analysis of EGF treated A431 cells using Akt (phospho-S473) antibody

Western blot analysis of A431 cells (Lane1), A431 cells (Lane2) using Akt (phospho-S473) antibody

Immunofluorescence analysis of A431 cells using Akt (phospho-S473) antibody

Western blot analysis of unstimulated NIH/3T3 cell lysates using Akt (phospho-S473) antibody