AKT antibody

• Catalog Number: orb345899
• Category: Antibodies
• Description: AKT antibody
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This AKT affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling.
• Concentration: 0.9 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This antibody is directed against AKT and reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT.
• Conjugation: Unconjugated
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store AKT antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: sheep anti-AKT Antibody, RAC-PK-alpha, Protein kin
• Note: For research use only
• Application notes: This Anti-AKT affinity purified antibody is tested for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user for IF and IHC. Expect a band approximately 55-60 kDa in size corresponding to AKT1 by western blotting in the appropriate cell lysate or extract. Cell Signaling, Neuroscience, Signal Transduction research.
• Expiration Date: 12 months from date of receipt.