AKT1 Antibody

SKU: orb345898

Description

AKT antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  This Anti-AKT affinity purified antibody is tested for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user for IF and IHC. Expect a band approximately 55-60 kDa in size corresponding to AKT1 by western blotting in the appropriate cell lysate or extract. Cell Signaling, Neuroscience, Signal Transduction research.

Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling.
• Target: AKT1
• Purity: This antibody is directed against AKT and reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Anti-AKT at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.9 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

sheep anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

Western Blot of Anti-AKT Antibody. Lane 1: HeLa cells (p/n orb348668). Lane 2: H2O2 treated MCF7 cells (p/n orb348670). Lane 3: Recombinant AKT. Lane 4: A431 cells. Load: 35 µg per lane. Primary antibody: AKT antibody at 1:300 for overnight at 4°C. Secondary antibody: IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1:10000 dilution for 45 min at room temperature. Block: 1% BLOTTO (B501) overnight at 4°C. Predicted/Observed size: ~55-60 kDa for AKT. Other band(s): unspecific.

Western Blot of Sheep Anti-AKT Antibody. Lane 1: Opal prestained Molecular weight markers. Lane 2: rec. AKT1 (p/n orb346473). Lane 3: rec. AKT2 (p/n orb346474). Lane 4: rec. AKT3 (p/n orb346475). Load: 50 ng. Primary antibody: AKT1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Donkey anti-Sheep IgG HRP (orb347959) at 1:70000 for 30 min at room temperature. Block: (p/n orb348644) BlockOut Buffer overnight at 4°C. Predicted/Observed size: ~60 kDa for AKT.

Quick Database Links

Gene Symbol

AKT1

UniProt

P31749

RefSeq:62241011