AKR1C4 Antibody

• Catalog Number: orb1247814
• Category: Antibodies
• Description: AKR1C4 Antibody
• Target: AKR1C4
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: DPKYQRVELNDGH-C
• UniProt ID: P17516
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 35kDa band observed in Human Liver lysates (calculated MW of 37.1kDa according to NP_001809.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows staining of distinct structures in the cytoplasm of hepatocytes. Recommended concentration, 2-4ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AKR1C4, aldo-keto reductase family 1, member C4, H
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_001809.2

orb1247814 (0.1 ug/ml) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247814 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.