AKR1C4 Antibody

SKU: orb1247814

Description

AKR1C4 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 35kDa band observed in Human Liver lysates (calculated MW of 37.1kDa according to NP_001809.2). Recommended concentration: 0.1-0.3µg/mL.Immunohistochemistry:In paraffin embedded Human Liver shows staining of distinct structures in the cytoplasm of hepatocytes. Recommended concentration, 2-4µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: DPKYQRVELNDGH-C
• Target: AKR1C4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AKR1C4, aldo-keto reductase family 1, member C4, HGNC:387, 3-alpha-HSD, C11, CDR, CHDR, DD4, HAKRA, MGC22581, 3-alpha hydroxysteroid dehydrogenase, type I , 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase 4, 3-alpha-hydroxysteroid dehydrogenase variant, aldo-keto reductase family 1, member C4, chlordecone reductase, dihydrodiol dehydrogenase 4, dihydrodiol dehydrogenase isozyme DD4, type I 3-alpha-hydroxysteroid dehydrogenase,

orb1247814 (0.1 ug/ml) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247814 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

AKR1C4

UniProt

P17516

RefSeq (Protein):NP_001809.2