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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IP, WB |
|---|---|
| Dilution Range | WB: 1:500-2000, IHC-P: 1:50-200 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C3 (NP_003730.4). |
| Molecular Weight | 37kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti DD3 antibody, anti DDX antibody, anti HA1753 antibody, anti HAKRB antibody, anti HAKRe antibody, anti HSD17B5 antibody, anti KIAA0119 antibody, anti hluPGFS antibody
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Immunofluorescence analysis of NIH/3T3 cells using AKR1C3 Rabbit pAb (orb48277) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 µg extracts of K-562 cells, using 3 µg AKR1C3 antibody (orb48277). Western blot was performed from the immunoprecipitate using AKR1C3 antibody (orb48277) at a dilution of 1:1000.
Western blot analysis of various lysates, using AKR1C3 Rabbit pAb (orb48277) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
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Rakhila, Halima et al. Identification of multiple and distinct defects in prostaglandin biosynthetic pathways in eutopic and ectopic endometrium of women with endometriosis Fertil Steril, 100, 1650-1659.e1-2 (2013)
Applications
IHC
Reactivity
Human
AKR1C3 Rabbit pAb (orb48277)
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