AKR1B1 Antibody (Center)

• Catalog Number: orb1931505
• Category: Antibodies
• Description: AKR1B1 Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P15121
• MW: 35853 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000, IF - 1:10-50, IHC-P - 1:100-500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ALDR1, ALR2 {ECO:0000303|PubMed:17368668
• Research Area: Metabolism Research, Signal Transduction
• Note: For research use only

Confocal immunofluorescent analysis of AKR1B1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35 ug/lane).AKR1B1 (arrow) was detected using the purified Pab.

Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.

All lanes: Anti-AKR1B1 Antibody (Center) at 1: 8000 dilution. Lane 1: A431 whole cell lysate. Lane 2: A549 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.