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Item 1 of 5
Item 1 of 5
AKR1B1 Antibody (Center)
Catalog Number: orb1931505
| Catalog Number | orb1931505 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB14877 |
| Tested applications | IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:10~50, WB: 1:8000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 35853 Da |
| Target | This AKR1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-135 amino acids from the Central region of human AKR1B1. |
| UniProt ID | P15121 |
| NCBI | NP_001619.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Aldose reductase, AR, Aldehyde reductase, Aldo-ket Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of AKR1B1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35 ug/lane).AKR1B1 (arrow) was detected using the purified Pab.
Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.
All lanes: Anti-AKR1B1 Antibody (Center) at 1: 8000 dilution. Lane 1: A431 whole cell lysate. Lane 2: A549 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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