AKR1B1 Antibody (Center)

SKU: orb1931505

Description

AKR1B1 Antibody (Center)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000, IF - 1:10-50, IHC-P - 1:100-500
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 35853 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

ALDR1, ALR2 {ECO:0000303|PubMed:17368668

Confocal immunofluorescent analysis of AKR1B1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35 ug/lane).AKR1B1 (arrow) was detected using the purified Pab.

Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.

All lanes: Anti-AKR1B1 Antibody (Center) at 1: 8000 dilution. Lane 1: A431 whole cell lysate. Lane 2: A549 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P15121