Cart summary

You have no items in your shopping cart.

AKR1B1 Antibody / Aldose reductase

Catalog Number: orb606701

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606701
CategoryAntibodies
DescriptionAKR1B1, also deisgnated as aldose reductase, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. It has also been shown to have decreased expression in adrenocortical cancer, and possibly play a role in adrenal tumorigenesis. It has been suggested that AKR1B1 could be investigated as a marker of malignancy for adrenal tumor diagnosis.
Species/HostMouse
ClonalityMonoclonal
Clone NumberCPTC-AKR1B1-2
Tested applicationsIF, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG1
ImmunogenRecombinant protein was used as the immunogen for this AKR1B1 antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 0.5-1ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis AKR1B1 antibody is available for research use only.
UniProt IDP15121
StorageStore the AKR1B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe optimal dilution of the AKR1B1 antibody for each application should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
AKR1B1 Antibody / Aldose reductase

IHC testing of FFPE human prostate with AKR1B1 antibody (clone CPTC-AKR1B1-2). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

AKR1B1 Antibody / Aldose reductase

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using AKR1B1 antibody (clone CPTC-AKR1B1-2). These results demonstrate the foremost specificity of the CPTC-AKR1B1-2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

AKR1B1 Antibody / Aldose reductase

SDS-PAGE analysis of purified, BSA-free AKR1B1 antibody (clone CPTC-AKR1B1-2) as confirmation of integrity and purity.

AKR1B1 Antibody / Aldose reductase

Western blot testing of human HeLa cell lysate with AKR1B1 antibody (clone CPTC-AKR1B1-2). Predicted molecular weight ~36 kDa.

AKR1B1 Antibody / Aldose reductase

Immunofluorescent staining of human A549 cells with AKR1B1 antibody (clone CPTC-AKR1B1-2, green) and DAPI nuclear stain (blue).

  • AKR1B1 Antibody / Aldose reductase [orb2641482]

    IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • AKR1B1 Antibody / Aldose reductase [orb1151447]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μg
  • AKR1B1 Antibody / Aldose reductase [orb1151720]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μg
  • AKR1B1 Antibody / Aldose reductase [orb606885]

    IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • AKR1B1 Antibody / Aldose reductase [orb2642443]

    IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg