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Catalog Number | orb1260347 |
---|---|
Category | Antibodies |
Description | AKR1A1 Antibody |
Target | AKR1A1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human AKR1A1 (NP_006057.1). |
UniProt ID | P14550 |
MW | Observed: 37kDa |
Tested applications | IF, IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | AKR1A1, aldo-keto reductase family 1, member A1 (a Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using AKR1A1 antibody (orb1260347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using AKR1A1 antibody (orb1260347) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using AKR1A1 antibody (orb1260347) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AKR1A1 antibody (orb1260347).
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |