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Catalog Number | orb764497 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to AKAP 79 |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human AKAP5. AA range:1-50 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 47 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-AKAP5 antibody, anti-AKAP79 antibody, anti-A- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using AKAP 79 Polyclonal Antibody diluted at 1:1000.
Immunofluorescence analysis of HeLa cells, using AKAP5 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2, HeLa, and COLO205 cells, using AKAP5 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).