Ajuba antibody

• Catalog Number: orb345492
• Category: Antibodies
• Description: Ajuba antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba.
• Concentration: 1.67 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:80,000, IP: 1:100, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human Ajuba. The product was affinity purified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, mouse and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Ajuba protein homologues from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q96IF1
• NCBI: 14249622
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Ajuba Antibody, Ajuba, JUB protein, aj
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size corresponding to AJUBA by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Ajuba-RFP fusion protein in cell lysates (arrow-head).

Western blot analysis of 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates using Ajuba antibody